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Figure 1 | Radiation Oncology

Figure 1

From: In vitro irradiation system for radiobiological experiments

Figure 1

Three-dimensional cell cultures. (a) MCTS growing in 50-mL vessels installed into the RCCS. Representative image of CAEP grown as (b, c) MCTS of about 1.3 -1.5 mm sampled from about 100 spherical colonies obtained from a single vessel or grown as (d, e) monolayer cultures with normal bright-field or phase contrast respectively, obtained by Olympus inverted microscope with attached Nikon high speed DS-Vi1 color digital camera. Spheroid number and size were determined through image analysis using NIS-Elements D software package.

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