Expression and purification of TAT-SOD
protein. (A) Schematic representation of the TAT-SOD1 protein. (B) Expressed and purified fusion proteins were analyzed by 12% SDS-PAGE followed by Coomassie Blue staining. Lanes in B are as follows: lane 1, non-induced TAT-SOD1; lane 2, induced TAT-SOD1; lane 3, supernatant after sonication; lane 4, precipitation after sonication; lane 5, Effluent after loading;lane6, washing solution; lane 7, eluent buffer 1; lane 8, eluent buffer 2. (C) Purified fusion proteins were subjected to immunofluorescence analysis with an anti-rabbit SOD1 antibody.