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Figure 4 | Radiation Oncology

Figure 4

From: Combined effects of C225 and 125-iodine seed radiation on colorectal cancer cells

Figure 4

C225 reduces the cellular DNA repair capacity. (A) Formation of γ-H2AX foci (red) in LS180 cells 48 h after the treatment; the nuclei were stained with Hoechst 33342 (blue); original magnification, ×20. (B) Percentage of γ-H2AX focus-positive cells at 48 h after the treatment. (C) Mean number of γ-H2AX foci per cell at 48 h. (D) Immunoblotting analysis of DNA-PK(cs), Ku80, Ku70, and actin (loading control) in LS180 cells at the indicated times after the treatment. Representative analysis of DNA-PKcs (E) and Ku70 (F) protein levels 48 h after the treatment. All data represent three independent experiments, mean ± SD. Unpaired t test was used for data analysis. ns (not significant) P > 0.05, *P < 0.05, **P < 0.01 as compared to 125I-CLDR group.

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