Figure 5

Cell apoptotic assay of CL187 cells after distinct modes of irradiation. A. In vitro assessment of apoptosis of CL187 cells. Percentage of apoptosis was measured by Annexin V and PI staining. The apoptosis was determined by FACS analysis of Annexin V-FITC and PI-staining 48 hrs after 0, 2, 4, 6 and 8Gy irradiation. Cells in the lower right quadrant indicate Annexin-positive, early apoptotic cells whereas the cells in the upper right quadrant indicate Annexin-positive/PI-positive, late apoptotic cells. B. Statistical analysis showed the percentages of early, late and total apoptosis of SDR, FDR and CLDR group 24, and 48 hrs after irradiation. The above data were one representative of three separate experiments, with three wells in each group. *p < 0.05. (show * in figures).