Figure 4

Tumor growth in mice following 10-Gy irradiation. The effect of IL-6 silencing vectors on tumor regrowth as demonstrated by (a) ectopic tumor growth curves and representative images 12 days after tumor implantation in pre-irradiated mice. (b) Effect of the IL-6–silencing vector on invasion was evaluated using the orthotopic tumor model. Representative images and quantitative data are shown. Y-axis represents the relative ratio, normalized to the number of TRAMP-C1 tumors developing extraprostatic extension 3 weeks after implantation in pre-irradiated mice.*, P < 0.05. (c) Expression levels of Ki-67, CD31, and VEGF were evaluated by immunofluorescence in growing tumors 12 days after implantation in irradiated mice.