Figure 4

Sequential read-out of Cell-Fit-HD. To avoid photoionization of FNTD crystals the imaging is first performed with the red laser on FNTD crystal and only after that the scan continues with blue laser on stained cell layer. The total axial range for the acquired image stack is about 120 μ m with depth increment (Δ z) of 3 μ m for FNTD crystal and Δ z= 0.3 μ m for the cell-layer. The axial range for the image stack of cells is about 10 μ m. The difference in optical sectioning is indicated by the striped arrows.