Ionizing Radiation (IR) upregulates AMPK expression and activity in A549 and H1299 lung xenografts in-vivo. ( A) Control and IR-treated tumours were subjected to immunoblotting analysis using AMPKα, P-AMPK (Thr172), and P-ACC antibodies. Anti-actin was used as a loading control. A representative immunoblot of 6 independent experiments is shown. ( B) Immunoblot densitometric values are shown as percent change in protein expression relative to the control group p (*p < 0.05; **p < 0.001). ( C) A549 and H1299 tumours were fixed and immunohistochemistry analysis was performed using a specific P-AMPK antibody.