Figure 7
Validation of the gene stability measure ( M ) and the geometric averaging of carefully selected control genes for normalization. The average gene-specific variation (determined as coefficient of variation in percent) for the three control genes with the smallest variation within each cell line after normalization with three different factors calculated as the geometric mean of the three control genes with the lowest (NF3(1–3)), intermediate (NF3(11–13)) and highest (NF3(30–32)) gene-stability values (as determined by geNorm). The data confirmed the stability of the ECGs. The value under the star indicates the normalization factor for each cell line.