Direct clustering of 32 ECGs expression. This Heat map represents expression of all 32 ECGs across the three cell lines (A431, A549 and BxPC3) irradiated with photon, proton and carbon ion. Genes were hierarchically clustered by Pearson correlation coefficient using average linkage. Green denotes genes with relatively decreased expression while red denotes genes with relatively increased expression. Scale bar represent log2 expression level of ECGs. C = Carbon, P = Proton, X = Photon and 0 = Control. Expression profile of A431 and A549 are similar as compared to BxPC3 which showed unique expression profile with less variations among the samples.