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Table 1 Discrimination of heterogeneity with the alkaline comet assay

From: Factors influencing heterogeneity of radiation-induced DNA-damage measured by the alkaline comet assay

    Paired comparison
Sample %Tail DNA SD Compared pair F-value p-value
0 Gy 6.2 7.6    
0 + 2 Gy 9.7 6.5 0 Gy / 0 + 2 Gy 24.03 <0.001
2 Gy 15.5 6.9 0 + 2 Gy / 2 Gy 74.31 <0.001
2 + 4 Gy 16.9 8.9 2 Gy / 2 + 4 Gy 3.220 0.073
4 Gy 20.7 8.1 2 + 4 Gy / 4 Gy 20.36 <0.001
4 + 8 Gy 30.1 12.3 4 Gy / 4 + 8 Gy 80.78 <0.001
8 Gy 43.1 14.4 4 + 8 Gy / 8 Gy 94.78 <0.001
  1. Leukocytes (200 leukocytes/sample of one donor) were irradiated with increasing doses (0-8 Gy) and mixed in equal proportions with the next higher irradiation dose level sample. Comparison of mean DNA damage in %Tail DNA for samples treated with 0, 2, 4 or 8 Gy and mixtures (0 and 2 Gy, 2 and 4 Gy, 4 and 8 Gy) revealed significant differences for almost all pairs and a trend for cells irradiated with 2 Gy vs. 2 + 4 Gy.
  2. Abbreviations: SD = standard deviation, p-value = probability value, F-value.