Skip to main content

Table 1 Discrimination of heterogeneity with the alkaline comet assay

From: Factors influencing heterogeneity of radiation-induced DNA-damage measured by the alkaline comet assay

   

Paired comparison

Sample

%Tail DNA

SD

Compared pair

F-value

p-value

0 Gy

6.2

7.6

   

0 + 2 Gy

9.7

6.5

0 Gy / 0 + 2 Gy

24.03

<0.001

2 Gy

15.5

6.9

0 + 2 Gy / 2 Gy

74.31

<0.001

2 + 4 Gy

16.9

8.9

2 Gy / 2 + 4 Gy

3.220

0.073

4 Gy

20.7

8.1

2 + 4 Gy / 4 Gy

20.36

<0.001

4 + 8 Gy

30.1

12.3

4 Gy / 4 + 8 Gy

80.78

<0.001

8 Gy

43.1

14.4

4 + 8 Gy / 8 Gy

94.78

<0.001

  1. Leukocytes (200 leukocytes/sample of one donor) were irradiated with increasing doses (0-8 Gy) and mixed in equal proportions with the next higher irradiation dose level sample. Comparison of mean DNA damage in %Tail DNA for samples treated with 0, 2, 4 or 8 Gy and mixtures (0 and 2 Gy, 2 and 4 Gy, 4 and 8 Gy) revealed significant differences for almost all pairs and a trend for cells irradiated with 2 Gy vs. 2 + 4 Gy.
  2. Abbreviations: SD = standard deviation, p-value = probability value, F-value.