Figure 3

DNA damage and heterogeneity. a) Mean variance and DNA damage in %Tail DNA measured after different experimental conditions (see Figure 1) were shown^*b) The reduction of heterogeneity by histones plus antioxidants and subsequent repair after 4 Gy was represented. Both conditions decreased heterogeneity of DNA damage to approximately one third of the initial level (fraction II i.e. pure DNA plus 4 Gy) ^*. c) The positive correlation (Pearson and Bravais’correlation, r = +0.880; p < 0.001) between DNA damage in %Tail DNA and variance of DNA damage was further substantiated by multiple linear regression (R² = 0.771) indicating a 77.1% dependency of variance by changes of DNA damage. The relationship between both parameters was visualised by a scatter graph (12 samples with 5 independent experiments, n = 60) ^*.^*200 leukocytes/sample of each donor were measured for calculation of DNA damage in %Tail DNA and variance. Mean variance was calculated from 5 donors.