Figure 6From: Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reducedDynamics of focal contacts and changes in the expression of cell surface integrins. Panels in (A) show AIR-induced changes of focal contacts by vinculin immunostaining, panels in (B) show FACS analyses of three different integrin subtypes (α2, β1, α5) in non-permeabilized CAFs from one representative donor (donor #1 in Table 1). In (C), mean and standard error of the fluorescent intensities of control (pale grey) and irradiated (dark grey) CAFs from three randomly selected donors (donors #1, #5, and #14) is presented. Intensities at irradiated conditions are relative-values of the non-irradiated and normalized control values, indicating X-fold enhancement of cell surface expression after 1 × 18 Gy. Statistical significance was set at p < 0.05 by applying Student's paired t-test. In panel (D), the total cellular pool of integrin subunit α2 (~150 kDa) was analysed by Western blots in whole cell lysates from two randomly selected donors.Back to article page