Dynamics of focal contacts and changes in the expression of cell surface integrins. Panels in (A) show AIR-induced changes of focal contacts by vinculin immunostaining, panels in (B) show FACS analyses of three different integrin subtypes (α2, β1, α5) in non-permeabilized CAFs from one representative donor (donor #1 in Table 1). In (C), mean and standard error of the fluorescent intensities of control (pale grey) and irradiated (dark grey) CAFs from three randomly selected donors (donors #1, #5, and #14) is presented. Intensities at irradiated conditions are relative-values of the non-irradiated and normalized control values, indicating X-fold enhancement of cell surface expression after 1 × 18 Gy. Statistical significance was set at p < 0.05 by applying Student's paired t-test. In panel (D), the total cellular pool of integrin subunit α2 (~150 kDa) was analysed by Western blots in whole cell lysates from two randomly selected donors.