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Figure 6 | Radiation Oncology

Figure 6

From: NVP-BEZ235 and NVP-BGT226, dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitors, enhance tumor and endothelial cell radiosensitivity

Figure 6

PI3K/mTOR inhibition attenuates tubule formation and migration by irradiated HUVEC and HDMVC. Cells were exposed to BEZ235 (50 nmol/L) for 1 h, before irradiation with 4 Gy. Cells were trypsinized immediately after irradiation and were incubated in basal medium containing 1.5% FCS and a constant concentration of VEGF (10 ng/ml). A, representative images of capillary tubule formation in HUVEC (left) and HDMVC (right) in response to BEZ235 (50 nmol/L). B, quantification of tubule formation as measured by the length of capillary-like tubular structures and normalized to the control group (4 power fields per sample). C, representative images of HUVEC (left) and HDMVC (right) migration 18 h post-irradiation. D, endothelial cell migration of indicated cells (treated as above). The number of migrated cells per power field (10X) was counted (12 fields per group) and normalized to the control group (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 over DMSO-treated control.

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Radiation Oncology

ISSN: 1748-717X

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