Skip to main content
Figure 1 | Radiation Oncology

Figure 1

From: NVP-BEZ235 and NVP-BGT226, dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitors, enhance tumor and endothelial cell radiosensitivity

Figure 1

BGT226 and BEZ235 attenuate oncogenic signaling and reduce clonogenic survival after radiation. SQ20B cells were exposed to the indicated drugs for 1 h and evaluated by Western blotting for phosphorylation of mTOR (Ser2448), Akt (Ser-473) and S6 protein (ser-235/236). β-actin was used as a loading control. A, Dose response of the PI3K/mTOR pathway in SQ20B cells after 1 h exposure to the indicated drug. B, Time-course of PI3K/mTOR inhibition to indicated drugs at maximal effective doses. C-D, Response of PI3K/mTOR pathway 1 h after 4 Gy. Cells were treated with 5 nmol/L BGT226 (C) or 50 nmol/L BEZ235 (D) for 1 h before and 1 h after irradiation. E, Plating efficiency of SQ20B,T24 and FaDu cells after exposure to indicated drugs for 1 h before up to 17 h post-irradiation (n = 3). F, Clonogenic survival of indicated cell lines after 18 h treatment with BEZ235 (50 nmol/L) and BGT226 (5 nmol/L), as described in "Methods" (n = 3). P < 0.05; **, P < 0.01 over DMSO-treated control.

Back to article page

Radiation Oncology

ISSN: 1748-717X

Contact us