Figure 4
The soluble form of ST6Gal I increases the migration of colon cancer cells. A. SW480 cells were transfected with WT ST6Gal I (WT) or ST6Gal I-ΔN (ΔN), and cell lysates were analyzed for the phosphorylated form of p130CAS (p-P130CASY410) and integrin β1 by immunoblotting. Sialylation of integrin β1 was determined by SNA lectin affinity assay. B. After transfection with the WT or ΔN form of ST6Gal I, cell migration was determined using Transwell migration assays. Upper: images of migrated cells captured by phase-contrast microscopy; lower: migration measured by counting cells. Data are presented as means ± SDs of three replicates (*p < 0.05 vs. the corresponding control).