Figure 2

Quantitative analyses of MDA concentration, SOD activity and relative mRNA levels of the antioxidant enzyme genes. (A) Relative MDA concentration in rat skin tissues of indicated groups. (B) SOD activity in rat skin tissues of indicated groups. (C) Representative reverse transcription (RT)-PCR analysis of the antioxidant enzyme genes in indicated groups. (D) Real-time PCR analysis of the antioxidant enzyme genes in indicated groups (4 animals per group). mRNA levels are presented as means ± SEM (normalized to β-Actin). The men expression level in PBS-injected control group was arbitrarily set as 100%. * P < 0.05; ** P < 0.01, compared with PBS-injected control group.