Methylation status was determined after exposure to DNMT inhibitors using Western blot analysis of DNMT1, 3A/3B. The cells were treated with DNMT inhibitors for 18 hours. A drastic depletion of DNMT1 and DNMT3A by psammaplin A, 5-aza-2'deoxycytidine, and zebularine in both A549 and U373MG cell lines was observed. However, there was no depletion of DNMT3B by the three DNMT inhibitors in either of the cell lines. Each blot is representative of two independent experiments, with actin used as a loading control.