Figure 5
From: Radiosensitization by the novel DNA intercalating agent vosaroxin
Mitotic catastrophe after irradiation increases at 72 hours with vosaroxin treatment. U251cells growing in chamber slides were exposed to vosaroxin (75 nmol/L) for 16 h, irradiated (2 Gy), and fixed at 24 h to 72 hours after irradiation for immunocytochemical analysis of mitotic catastrophe. Nuclear fragmentation (defined as the presence of two or more distinct lobes within a single cell) was evaluated in at least 150 cells per treatment per experiment. * = p < 0.05 comparing cells in the combination group compared to either drug or radiation alone groups at the same time point.