Figure 5

The interrelationship between autophagy and apoptosis in SKOV3 and SKVCR. Apoptosis and autophagy were determined in the presence or absence of 3MA or ZVAD, with or withour indicated IR regimens at 24 h. (A, C) Flow cytometry was used to quantitative the apoptotic rate in SKOV3 and SKVCR. (B, D) Statistical analysis of apoptotic rate Results were expressed as the percentage of untreated cells, Mean ± SD of thrice. *P<0.05, vs untreated cells. (E) Western blot was used to detect caspase 3 and cleaved caspase 3 in SKVCR, the increase of cleaved caspase 3 suggested the apoptosis occurrence. (F,H) MDC staining was used to detect the morphologic changes of autophagy in SKOV3 and SKVCR. (G,I) Statistical analysis of autophagic rate, results were expressed as the percentage of untreated cells, Mean ± SD of thrice. *P<0.05, vs untreated cells.