Figure 1
Vorinostat, hypoxia, and ionizing radiation (IR) – in vitro clonogenicity and HIF-1α expression. (A) HCT116, (B) HT29, KM20L2 and SW620 cells were treated with IR under normoxia (IR-NO), IR under hypoxia (IR-HO), vorinostat and IR under normoxia (VOR-IR-NO) or vorinostat and IR under hypoxia (VOR-IR-HO). Surviving fractions are shown as mean, and bars represent SEM. * Indicates significant difference between IR-NO and IR-HO. ** Indicates significant difference between VOR-IR-NO and IR-NO. *** Indicates significant difference between VOR-IR-HO and IR-HO. (C) Western immunoblot analysis of HIF-1α expression in HCT116 cells exposed to normoxia (NO) or hypoxia (HO). Cells were treated with CoCl2 as a positive control and actin was used as protein loading control.