Figure 4From: Preclinical evaluation of sunitinib, a multi-tyrosine kinase inhibitor, as a radiosensitizer for human prostate cancerThe effects of sunitinib on downstream signals. Both DU145 (A) and PC3 (B) cells were treated with sunitinib (40, 100, 250 nM) and harvested for western blot analysis 2 hours after irradiation (5 Gy). Cells were assessed for the effect of sunitinib on two key signaling proteins, ERK and AKT; both of which are involved in the radiation response and lie downstream of all three RTK targets, p-PDGFR-β, p-VEGFR2, and p-C-KIT. Actin was used as a loading control.Back to article page