Figure 1

NO production of RAW 264.7 cells stimulated by LPS. A. RAW cells were exposed to either 0 ng/mL or 30 ng/mL LPS. At scheduled time points, the cell supernatant was collected for determination of NO₂^- with Griess reagent. B. RAW cells were exposed to LPS for 48 h at different concentrations, then NO₂^- was measured in the same way as in A. Values are averages ± SD of two independent experiments each done in triplicates; (**) indicates p < 0.01, (one way ANOVA).