Affinity of CG-HVJ-E for tumor cells and the intracellular uptake of molecules incorporated into HVJ-E. A) Affinity of HVJ-E and CG-HVJ-E for tumor cells. LM8G5 cells were incubated alone (a), or with Qdot (b), HVJ-E-Qdot (c), or CG-HVJ-E-Qdot (d) for 60 min in a Lab-tek chamber slide and examined for Qdot (red) and Hoechst 33342 (blue) by fluorescence microscopy, Representative views are shown. B) Intracellular localization of Qdot transported by CG-HVJ-E. Tumor cells were incubated with CG-HVJ-E-Qdot (orange) and stained with Hoechst 33342 (blue) and Alexa Fluor 488 phalloidin (green). Image shows 3-dimensional analysis with confocal microscopy. C) Luciferase activity in tumor cells transfected with HVJ-E or CG-HVJ-E. Cells were cultured for 30 min with HVJ-E or CG-HVJ-E containing a luciferase-expressing plasmid. Luciferase activity was measured 24 hours later to evaluate the transfection efficiency. Results are shown as means ± SD (n = 4). Similar results were obtained in three experiments. * p < 0.05. D) 10B accumulation and retention in tumor cells in vitro. Cells were incubated with 20 μg boron/ml of BSH or CG-HVJ-E-BSH for 30 min, then washed twice with PBS, and the 10B concentration was measured by ICP-AES. Separately, cells were incubated in the same manner, but after washing, were incubated in medium without BSH for 24 or 48 hours before testing for 10B concentration as described above. The horizontal axis shows time after co-incubation. The vertical axis shows the percent of the administered dose (% dose) of CG-HVJ-E-BSH (open diamond) or BSH (solid square). Results shown are the means ± S.D. (n = 3). * p < 0.05.