Figure 2

Affinity of CG-HVJ-E for tumor cells and the intracellular uptake of molecules incorporated into HVJ-E. A) Affinity of HVJ-E and CG-HVJ-E for tumor cells. LM8G5 cells were incubated alone (a), or with Qdot (b), HVJ-E-Qdot (c), or CG-HVJ-E-Qdot (d) for 60 min in a Lab-tek chamber slide and examined for Qdot (red) and Hoechst 33342 (blue) by fluorescence microscopy, Representative views are shown. B) Intracellular localization of Qdot transported by CG-HVJ-E. Tumor cells were incubated with CG-HVJ-E-Qdot (orange) and stained with Hoechst 33342 (blue) and Alexa Fluor 488 phalloidin (green). Image shows 3-dimensional analysis with confocal microscopy. C) Luciferase activity in tumor cells transfected with HVJ-E or CG-HVJ-E. Cells were cultured for 30 min with HVJ-E or CG-HVJ-E containing a luciferase-expressing plasmid. Luciferase activity was measured 24 hours later to evaluate the transfection efficiency. Results are shown as means ± SD (n = 4). Similar results were obtained in three experiments. * p < 0.05. D) ¹⁰B accumulation and retention in tumor cells in vitro. Cells were incubated with 20 μg boron/ml of BSH or CG-HVJ-E-BSH for 30 min, then washed twice with PBS, and the ¹⁰B concentration was measured by ICP-AES. Separately, cells were incubated in the same manner, but after washing, were incubated in medium without BSH for 24 or 48 hours before testing for ¹⁰B concentration as described above. The horizontal axis shows time after co-incubation. The vertical axis shows the percent of the administered dose (% dose) of CG-HVJ-E-BSH (open diamond) or BSH (solid square). Results shown are the means ± S.D. (n = 3). * p < 0.05.