Skip to main content
Figure 7 | Radiation Oncology

Figure 7

From: Delayed cell death associated with mitotic catastrophe in γ-irradiated stem-like glioma cells

Figure 7

Correlation of proliferation with γIR-induced mitotic catastrophe and late apoptosis in SLGCs. A. Analyses of CD133+ and CD133- immature glioma cells. Flow cytometry analysis of CD133 expression in magnetic bead-separated CD133+/CD133-populations of GBM8 SLGCs (upper left). Expression of CD133, Sox2, musashi and nestin as determined by Western blotting (upper right). Viable and dead cells (lower left) and cells with signs of mitotic catastrophe (lower right) 7 d after irradiation. B. Influence of EGF plus FGF-2 on proliferation, mitotic catastrophe, and apoptosis of GBM4 SLGCs. The cells were incubated with or without EGF plus FGF-2 (20 ng/ml). After 24 h the cells were irradiated, and 7 d later analyzed. In the cytokine-treated cultures, EGF and FGF-2 were replenished after 3 days. Mean ± S.D. of at least three experiments is shown; statistical significance (p < .05).

Back to article page