Figure 5

γ IR-induced mitotic catastrophe and cell cycle arrest in SLGCs. SLGCs and FBS cultures were irradiated with 0, 2, 5, or 10 Gy, and DAPI-stained cells with signs of mitotic catastrophe (micro- and multinucleated cells) were detected microscopically (A) and counted (B) at the time points indicated. C. γIR-induced cell cycle arrests in SLGC cultures. Exponentially growing cells were irradiated with 10 Gy and 24 h later cell cycle analysis was performed by flow cytometry. The percentages of cells in the different cell cycle phases are indicated. In each panel, one of two experiments with similar results is shown.