Figure 1

Characterization of SLGCs and FBS cultures derived directly from the SLGCs. A. Sphere formation 14 days after seeding 500 cells/well in 24 well plates. B. Immunofluorescence analysis of neural stem- and progenitor markers (Sox2, CD133, musashi, nestin) and differentiation markers (GFAP, Tuj) in SLGC and in differentiating FBS cultures. Nuclei were counterstained with DAPI. C-E. Western blot analysis of stem- and progenitor markers of SLGCs differentiating in FBS-containing medium (C), of SLGCs resistant to differention in FBS-containing medium (D) but differentiating after exposure to vitamin A (E). Blots shown are representative of at least three independent experiments. The analyses were performed after culturing for 4 weeks under differentiating conditions.