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Figure 2 | Radiation Oncology

Figure 2

From: Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response

Figure 2

The effect of BO-1051 on tumor cell radiosensitivity. Cultures of (A) U87MG, (B) U251MG and (C) GBM-3 cells were exposed to 200 nM of BO-1051 or DMSO (IR only) for 24 h and irradiated with graded doses of γ-rays, rinsed, and fed with fresh growth media. Colony-forming efficiency was determined 10-14 days later, and survival curves were generated after normalizing for cell killing by BO-1051 alone. Points: mean survival fraction from at least 3 independent experiments; bars: SD. (D) The survival fraction after 2 Gy (SF2), corrected for independent cytotoxic effect of BO-1051, of human glioma cells treated with 200 nM of BO-1051 or control (DMSO) for 24 h pre-radiation was measured. Values are the mean survival fraction ± SD of at least 3 independent experiments. * p < 0.05. (E) Sensitizer enhancement ratios (SER) of human glioma cells. SERs were calculated at 10% or 50% cell survival (0.1 or 0.5) by dividing the dose of radiation from the radiation-only surviving curve with the corresponding dose from the BO-1051 plus radiation curve.

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