Figure 2From: Biological in-vivo measurement of dose distribution in patients' lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate glandThe calibration curve was set-up by irradiating blood samples of five volunteers and is used to correlate the delivered dose with the mean number of induced gamma-H2AX foci per nucleus, scored 2 hours after irradiation. Background foci levels were subtracted. Lymphocytes were irradiated ex vivo at six different doses (0 - 2Gy) in three independent measurements each (standard deviations are shown).Back to article page