Figure 3

Resveratrol (RSV) enhances ionizing radiation (IR)-induced cell death. Induction of cleaved caspase-3 and nuclear aberrations. A. PC3 cells were pre-treated or not with RSV (5 μM) for 1 hour after which they were treated with IR (8 Gy). Cells were lysed 1 hour after IR exposure and subjected to immunoblot analysis using the indicated antibodies. Shown are representative immunoblots of 3-4 independent experiments. B. and C. Cells were treated or not with RSV (5 μM) prior to IR treatment (8 Gy) followed by staining with nuclear stain Hoeschst 33258 (low magnification, in B) or Hoeschst 33258 and immunostaining of tubulin (high magnification, in C) used to illustrate better cellular morphology. Morphology was assessed using fluorescence microscopy. Representative images from 4 independent experiments are shown. D. Average ± SE values (% Nuclear Aberrations: as indicated by the proportion of cells with features of nuclear fragmentation, micro-nuclei and multi-nucleation) were extracted through quantitation of 4 independent experiments (in B), as described in Methods.