Figure 2From: Resveratrol enhances prostate cancer cell response to ionizing radiation. Modulation of the AMPK, Akt and mTOR pathwaysEffects of resveratrol (RSV) and ionizing radiation (IR) on prostate cancer cell cycle progression and expression of cycle regulators. A. 22RV1 cells were pre-treated or not with RSV (5 μM) for 1 hour followed by treatment with 8 Gy IR. Cells were allowed to cycle for 48 hours after which they were fixed, treated with a triton-X and stained with propidium iodide (PI) solution as described in Methods. Shown are representative results of 3 independent experiments. Pie charts illustrate the proportion of cells in each phase of cell cycle. B. 22RV1 and PC3 cells were pre-treated with RSV (5 μM) for 1 hour followed by 8 Gy IR treatment. Cells were lysed 1 hour after IR exposure and subjected to immunoblot analysis using antibodies against the indicated cell cycle markers. Shown are representative immunoblots of 3 independent experiments.Back to article page