Table 1 The primer sequences and annealing temperatures for the seven genes studied
Gene | Annealing temperature(°C) | Primer sequence | Amplicon (bp) | Accession No. | |
|---|---|---|---|---|---|
β-actin | 60 | Forward Reverse | ATCGTGCGTGACATTAAGGAGAAG AGGAAGGAAGGCTGGAAGAGTG | 179 | NM_001101 |
β1-AR | 60 | Forward Reverse | GGGAGAAGCATTAGGAGGG CAAGGAAAGCAAGGTGGG | 270 | NM_000684 |
β2-AR | 60 | Forward Reverse | CAGCAAAGGGACGAGGTG AAGTAATGGCAAAGTAGCG | 334 | NM_000024 |
COX-2 | 57 | Forward Reverse | TTGACCAGAGCAGGCAGATG CCAGAAGGGCAGGATACAGC | 171 | NM_000963.2 |
VEGF-A | 57 | Forward Reverse | CTGGGCTGTTCTCGCTTCG CTCTCCTCTTCCTTCTCTTCTTCC | 140 | NM_001025370.1 |
EGFR | 53 | Forward Reverse | AGG ACA GCA TAG ACG ACA C AGG ATT CTG CAC AGA GCC A | 90 | NM_005228.3 |