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Table 2 Primers for quantitative real-time RT-PCR

From: Effects of osteopontin inhibition on radiosensitivityof MDA-MB-231 breast cancer cells

gene

primer

sequence 5'→3'

 

localization

HPRT

HPRT fw

5'-TTGCTGACCTGCTGGATTAC-3'

sense

309-328

 

HPRT rev

5'-CTTGCGACCTTGACCATCTT-3'

antisense

551-570

OPN

OPN fw

5'-TGGCCGAGGTGATAGTGTG-3'

sense

555-573

 

OPN rev

5'-CGGGGATGGCCTTGTATG-3'

antisense

686-703

  1. Primer sequences and the localization of the primer binding side in the corresponding mRNA transcript