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Figure 3 | Radiation Oncology

Figure 3

From: Effects of osteopontin inhibition on radiosensitivityof MDA-MB-231 breast cancer cells

Figure 3

PARP protein levels and apoptosis rate of either non-irradiated or irradiated cells after siRNA transfection. A. Western blot analysis of PARP with rabbit anti-human cleaved PARP (Asp214) antibody [1] in MDA-MB-321 cells 24 h and 72 h after transfection. The cells were untreated (UT), transfected with 100 nM of either nonsense siRNA (non) or target-specific siRNAs to knockdown OPN (Mix and OpnS). The Western blot shows one representative result out of three independent experiments. Actin served as an internal loading control. B. The morphology of DAPI stained cell nuclei was analyzed to quantify the apoptosis rate of MDA-MB-231 cells 72 h after transfection. The diagram shows the apoptosis rate of the cells as a function of treatment and irradiation. A fluorescence microscope was used and 500 cells in several fields of view were counted for each experiment. Data represent the average values (± SD) of three independent experiments (* p < 0.05, ** p < 0.001).

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