Figure 2
From: Effects of osteopontin inhibition on radiosensitivityof MDA-MB-231 breast cancer cells
Migration behavior of either non-irradiated or irradiated (2 Gy) MDA-MB-231 cells after siRNA transfection. A. Scratch assay: Wound scratch assay of MDA-MB-231 cells 24 h after transfection. Untreated cells and cells that were treated with nonsense siRNA were able to close the wound scratch by migration. Cells treated with Mix as well as OpnS did not migrate and were unable to close the wound scratch. B. Boyden chamber assay: The migration rate of cells treated with OPN-specific siRNAs was normalized to migration rate of cells treated with nonsense siRNA. Treatment with siRNAs targeting OPN reduced the migration rate in non-irradiated cells as well as in cells irradiated at 2 Gy. The migration rate of cells transfected with nonsense siRNA at 0 Gy was arbitrarily established as 100%. Data represent the average values (± SD) of three independent experiments (* p < 0.05).