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Figure 1 | Radiation Oncology

Figure 1

From: Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

Figure 1

Screening and characterization of positive phage clones of anti-DNA-PKcs segments scFv. (A) Conserved functional domains of DNA-PKcs and the location of segments identified with epitopes predicted in DNA-PKcs. DPK1, DPK2 are located at the N-terminus of DNA-PKcs, and DPK3 and DPK4 are located between the leucine zipper (LZ) and T2609 autophosphorylation cluster. The DPK3 segment includes Ser2056 autophosphorylation cluster. (B) PAGE electrophoresis patterns of the Mva I-digested positive anti-DPK3-scFv clones. (C) Coomassie brilliant blue stained gel of the SDS-PAGE analysis of 10 μg purified DPK3 (~30 kD) and DPK4 (~32 kD) segments. (D) Immunohybridization (western blotting) analysis of purified DPK3 (30 kD) and DPK4 (32 kD) segments using anti-DPK3-scFv-2 antibody. (E) Immunohybridization analysis of DNA-PKcs in HeLa cell protein lysate using anti-DPK3-scFv-2 and anti-DNA-PKcs antibodies.

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