Figure 6

DCQ modulates the protein expression levels of key mediators of apoptosis and mitotic catastrophe. At 50% confluency, cells were treated with 5 or 10 μM DCQ for 6 hrs. Whole cell lysates were then immunoblotted with the different primary antibodies and probed with GAPDH to ensure equal loading. (A) p53 and p21 protein expression and (B) caspase-2 and PIDD-C protein expression in HCT116 cell lines in response to DCQ treatment under normoxic or hypoxic conditions. (C) Relative densitometry values of analyzed proteins are plotted. All values were normalized to GAPDH and calculated relative to the control cells cultured under normoxia. The experiment was repeated twice each in duplicates.