Figure 3

Caspase independent inhibition of CGK733 on MCF-7 proliferation. A. Cells were cultured with the indicated doses of CGK733 ± pan- caspase inhibitor z-VAD-fmk (40 μM) for 48 h. Cell proliferation was measured as described in Materials and methods. Results represent the mean ± S.E. from three independent experiments. B. MCF-7 cells were grown on coverslips and treated with 10 μM CGK733 or 10 μM KU55933 for 48 h. Cells were fixed and stained with DAPI as described in Materials and methods. White arrows denote condensed nuclei. Scale bar 10 μm.