Figure 4
From: Radiation-induced Akt activation modulates radioresistance in human glioblastoma cells
Expression of PTEN increases the radiosensitivity of U87MG cells. A. Genetically modified U87MG cells were treated with vehicle or 1 μg/mL doxycycline for 24 hr before harvest. Cell lysates were subjected to Western blot analysis with indicated antibody. B. Genetically modified U87MG cells were treated with 1 μg/mL doxycycline for 24 hr, and then irradiated with the indicated does. Afterwards, cells were incubated for 4 hr at 37°C, and trypsinized and seeded for clonogenic assay. Colony-forming efficiency was determined 14 d later.