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Figure 3 | Radiation Oncology

Figure 3

From: Radiation-induced Akt activation modulates radioresistance in human glioblastoma cells

Figure 3

Inhibition of PI3K-Akt signaling with PI3K inhibitor LY294002 or EGFR inhibitor AG1478 increases the radiosensitivity of U87MG cells. A. U87MG cells were treated with 20 μM LY294002 for 1 hr prior to IR, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control. B. Cells were treated with vehicle (control) or 20 μM LY294002 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assays. Colony-forming efficiency was determined 14 d later. Results were pooled from three different experiments. C. Cells were treated with vehicle (control) or 5 μM AG1478 for 16 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.

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