Figure 7

Cell cycle distribution of PC-3 cells. At point "0 h" serum was withdrawn. At point "24 h" incubation genistein 10 μM was added to designated probes. At point "48 h" designated probes were irradiated with 4 Gy. In the following time, every 6 h probes were stained with DAPI and analyzed in a flow cytometer up to point "72 h". Every result reflects 3 independent assays. Proportion of cells in G0/G1 is symbolised by a bar, G2/M by a white bar and S-phase by a black bar. In the controls 15% of the cells were in S-phase, 65% in G0/G1 and 20% in G2/M. In the following, the S-phase was reduced to 5%, while G0/G1 increased to 73%. The proportion of cells in G2/M showed minimal changes. After incubation with genistein 10 μM no significant differences when compared to untreated controls were observed. After irradiation with 4 Gy the proportion of cells in G0/G1 decreased from 73% to 62%, in S-phase decreased from 8% to 3.6% and in G2/M phase increased from 21% to 34.3%. Similar results were observed after incubation with genistein 10 μM and irradiation with 4 Gy. In the controls 15% of the cells were in S-phase, 65% in G0/G1 and 20% in G2/M. In the following, the S-phase was reduced to 5%, while G0/G1 increased to 73%. The proportion of cells in G2/M was only minimal changes. After incubation with genistein 10 μM no significant differences when compared to untreated controls were observed. After irradiation with 4 Gy the proportion of cells in G0/G1 decreased from 73% to 62%, in S-phase decreased from 8% to 3.6% and in G2/M phase increased from 21% to 34.3%. Similar results were observed after incubation with genistein 10 μM and irradiation with 4 Gy.