Figure 1

Immuncytological staining of ER-α and -β in EFO-21 (positive control), BG-1 (negative control), LNCaP and PC-3. In the first row ER-α has been stained, in the second ER-β. EFO-21 and BG-1 cells served as controls: EFO-21 is an ovarian carcinoma cell line that expresses both ER-α and ER-β, whereas BG-1 is an ovarian cell that does not express these receptors. Expression of the receptors reflects a brown staining. For easier analysis, staining of the nuclei with DAB was not performed in the presented samples of LNCaP and PC-3. While LNCaP cells showed expression of ER-α and ER-β, PC-3 cells did not.