Figure 6

Clonogenic survival of LNCaP cells after incubation with goserelin 0.01 μM and 10 μM 12 h before and after irradiation with single doses between 0 and 4 Gy. Colonies were evaluated after 6 cell doublings (only colonies > 50 cells counted). Survival was expressed relative to sham-irradiated controls. Experiments were performed in quadruplicate and repeated at least three times. Error bars represent standard errors. Linear-quadratic equation was used for the control and the goserelin 10 μM curve, a polynominal equation for the goserelin 0.01 μM curve. Incubation with hormones in different concentrations (dotted lines) did not alter cell survival significantly when compared to untreated controls.