Figure 4

Viability of PC-3 cells 12 h, 36 h, and 60 h after irradiation (t = 0 h) with 0 Gy (A), 1 Gy (B), 2 Gy (C) and 4 Gy (D) and incubation with different concentrations of goserelin (from 12 h before irradiation on). Experiments were performed in triplicate and repeated at least three times. At the different time-points the cells were incubated with CTB-reagent for 4 h, than absorption was measured at 570 nm and 620 nm. After blank-reduction radiation the results were normalized to the specific irradiation controls (without goserelin). Results are expressed as measured absorbance. Error bars represent standard deviations. The effect of irradiation alone is shown in figure E. In PC-3, neither incubation with goserelin nor the combination of goserelin and irradiation showed a significant reduction in cell viability when compared to the particular controls.