Figure 2

Western Blot of c-fos, phosphorylated p38 and phosphorylated ERK1/2 after incubation with goserelin and irradiation with 4 Gy. Cells were incubated with HFM for 24 h, than 10 μM goserelin was added. 12 h later the flasks were irradiated with 4 Gy and after 12 h the cells were trypsinized. In LNCaP incubation with goserelin induced the expression of c-fos more than irradiation alone. In PC-3 the expression of c-fos was not changed. Phosphorylated p38 was induced by goserelin incubation and irradiation in LNCaP. In PC-3, there was a higher expression of this protein after incubation with goserelin than after irradiation alone. Phosphorylated ERK1/2 was induced after goserelin incubation and irradiation in LNCaP, but not in PC-3. These results show, that the incubation with goserelin was functionally active in the tested cell systems.