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Figure 5 | Radiation Oncology

Figure 5

From: Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition

Figure 5

Cell cycle regulatory factors upon TSA treatment. The HCT116 and SW620 cell lines were treated (+) with 100 nM TSA, or left untreated (-), and further incubated for the indicated time periods before analysis. Upper panel: Protein expression levels of Plk1, p53, Cyclin D1, and p21 were analyzed by Western blot immunostaining, using α-tubulin as protein loading control. Lower panel: mRNA expression levels of PLK, CCND1, and CDKN1A were analyzed by Northern blot hybridization, using 18S rRNA as RNA loading control.

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