MVP and vaults: a role in the radiation response
© Lara et al; licensee BioMed Central Ltd. 2011
Received: 3 August 2011
Accepted: 31 October 2011
Published: 31 October 2011
Vaults are evolutionary highly conserved ribonucleoproteins particles with a hollow barrel-like structure. The main component of vaults represents the 110 kDa major vault protein (MVP), whereas two minor vaults proteins comprise the 193 kDa vault poly(ADP-ribose) polymerase (vPARP) and the 240 kDa telomerase-associated protein-1 (TEP-1). Additionally, at least one small and untranslated RNA is found as a constitutive component. MVP seems to play an important role in the development of multidrug resistance. This particle has also been implicated in the regulation of several cellular processes including transport mechanisms, signal transmission and immune responses. Vaults are considered a prognostic marker for different cancer types. The level of MVP expression predicts the clinical outcome after chemotherapy in different tumour types. Recently, new roles have been assigned to MVP and vaults including the association with the insulin-like growth factor-1, hypoxia-inducible factor-1alpha, and the two major DNA double-strand break repair machineries: non-homologous endjoining and homologous recombination. Furthermore, MVP has been proposed as a useful prognostic factor associated with radiotherapy resistance. Here, we review these novel actions of vaults and discuss a putative role of MVP and vaults in the response to radiotherapy.
Keywordsmajor vault protein radiotherapy prognosis radiation response
Major vault protein: an overview of structure and composition
The sequences of the 2 other proteins, which are not part of this shell-like structure and probably reside at the top center of the caps or within the vaults, are identified and are present also in the human genome. VPARP presumably ribosylates substrates and TEP1 is important for stabilization of vRNA. Molecular composition of the vault has been roughly estimated as 78-96 MVPs, eight VPARPs, two TEP1s, and at least six copies of vRNA . Both the high degree of evolutionary conservation and the complex structure of vault particles, as well as its broad distribution in tissues, suggest an important function in cellular processes . Although vaults have been proposed to play a role in drug resistance, nucleocytoplasmic transport, and regulation of signaling, a definitive function for MVP or vaults has yet to be assigned as MVP knockout mice (MVP-/-) do not have phenotypes consistent with these in vitro observations . This suggests that even though the major component of the vault particle is absent in MVP-/--mice, and vault particles are no longer detected, the remaining components TEP1, VPARP, and vRNA might still interact and possibly fulfill a functional role.
The human gene encoding MVP has been located in chromosome 16 (16p11.2) , approximately 27 cM proximal to the gene location of the multidrug resistance protein-1 (MRP1, also designated as ABCC1) . However, although both the ABCC1 and MVP maps to the short arm of chromosome 16, they are rarely coamplified and are normally not located within the same amplicon and can be switched on separately [12, 13].
Analysis of the human MVP gene revealed a TATA-less promoter, which also lacks other core-promoter elements but harbors several putative transcription factor binding sites, including an inverted CCAAT box, a p53-binding site, and a GC box element . In silico analysis identified a putative STAT-binding site that strongly resembles an interferon-γ-activated site element (GAS), which binds preferentially to STAT1 homodimers . Disruption of the STAT-binding site reduces basal MVP promoter activity, suggesting a role of JAK/STAT signals in the activation of MVP expression . With up to 105 particles per cell, vaults are abundantly present in many different cell types, although its expression varies among tissues. Vaults are most numerous in macrophages [17, 18] and epithelial cells with secretory and excretory functions as well as cells chronically exposed to xenobiotics such as bronchial cells and cells lining the intestine . Most of the vaults are located in the cytoplasm, although a small fraction of these particles apparently is also localized at or near the nuclear membrane and the nuclear pore complex [20, 21]. The widespread of vaults in many diverse organisms and cell types suggests that their function is essential to eukaryotic cells.
Interaction Partners and Putative Functions of Vaults
The detailed insight on the structure of MVP and vaults contrasts with the poor understanding of their endogenous function. Several observations though indicate that MVP is directly involved in major cytoplasmic signal transduction cascades. In EGF-stimulated cells, tyrosine-phosphorylated MVP complexes with the SH2-domain-containing tyrosine phosphatase SHP-2 and interacts with ras-activated downstream kinase Erk, presumably to fine-tune the activity of the Erk-downstream target Elk-1 . But tyrosine-phosphorylated MVP also binds to the SH2-domain of Src-kinase and downregulates EGF-dependent ERK activation in Src overexpressing cells . Likewise MVP also binds to the tumour suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) and a significant quantity of endogenous PTEN associates with vault particles in HeLa cells, thus suggesting a regulatory role for MVP in the phosphoinositide 3-kinase/Akt (PI 3-kinase/Akt) signaling pathway . However, MVP-PTEN-interaction might only be relevant for nuclear import of PTEN thereby regulating the nuclear function of PTEN (, see below). Eventually, the identification of the specific growth factor-regulated MVP phosphorylation sites and the complexed interaction partner on MVP in response to growth factor stimulation are required to reveal a scaffold or direct regulatory function of MVP/vaults on these signal transduction cascades.
Likewise, a co-immunoprecipitation approach revealed that the UV-induced phosphorylation status of MVP affects the capacity of the constitutive photomorphogenic 1 ubiquitin ligase (COP1) to interact with the transcription factor c-jun. In unstressed cells, cytoplasmic MVP/vault binds to and modifies COP1, presumably via the other vault components. Following shuttling into the nucleus, COP1 complexes with c-jun with high affinity and suppresses its transcriptional activity by targeting c-jun for degradation. After UV irradiation, phosphorylated MVP does not interact with COP1 anymore, eventually leading to the alleviation of COP1-c-jun- complex formation. Free c-jun subsequently drives AP-1-controlled gene expression . Negative regulation of downstream signaling has also been identified on the level of the hypoxia-inducible factor-1α (HIF-1). HIF-1α and MVP-expression are increased under hypoxia. However, the stability of HIF-1α protein is enhanced to higher extents in MVP-knockdown cells. Direct complex formation of vaults with HIF-1α, PHDs and pVHL was demonstrated and suggests that MVP/vault might act as scaffold protein for ubiquitination and degradation of HIF1α  and thereby affects hypoxia-regulated expression of HIF-1α-dependent gene expression.
The generation of MVP-/--mice did not reveal any further indications on an essential role of MVP and vaults during embryogenesis and in the cellular processes in tissues of adult mice under normal environmental conditions. Furthermore, both embryonic stem cells and bone marrow cells derived from MVP-/--mice display a similar sensitivity towards classic cytostatic agents as their wildtype counterpart cells, and normal tissue toxicity towards doxorubicin in vivo is unchanged in the MVP-deficient mice. Interestingly though and unexpectedly identified, MVP and vaults are part of the effective innate immune response and contribute to the uptake and clearance of lung pathogens such as Pseudomonas aeruginosa via lipid rafts in lung epithelial cells, and eventually resistance to lethality . Furthermore, the activation of MVP-expression by interferon γ through the JAK/STAT pathway and enhanced expression of MVP in macrophages and in dendritic cells suggest a clear relationship with the immune system [16, 29]. However, despite the high abundance of MVP/vaults in MVP-wildtype dendritic cells, no differences in dendritic cell migration and antigen presentation and T-cell responses could be observed in MVP-/--mice. In human beings, a strong up-regulation of vault expression during dentritic cell culturing was observed, proposing that vaults act as components in the cascade of events regulating dentritic cell effector function . Besides the activation of MVP by interferon, several other observations indicate that MVP might be involved in immunological responses . It has been reported a dramatic induction of vRNA expression in response to infection with Epstein-Barr virus [32, 33], and vRNA induction has been has been suggested as playing important roles in innate immunity . This led to speculate that vRNA/vaults could be involved in anti-viral defence.
Finally, the hollow structure, the rapid movements, the distinct subcellular localisation (as for example at the nuclear membrane), and the in vitro and clinical correlations with drug resistance in several types of human cancers led to the hypothesis that vaults might represent rather promiscuous transport vehicles [35–37].
Drug resistance: the best known role of MVP
The MVP has originally been identified as the lung resistance protein . LRP was discovered almost 20 years ago as a protein overexpressed in a multidrug-resistant lung cancer cell line diverting cytostatic drugs from intracellular targets and conferring multidrug resistance (MDR) . Several proteins have been linked to MDR, such as the ABC-drug transporter p-Glycoprotein (P-gp, also designated as ABCB1), members of the multidrug resistant protein family (ABCC1, ABCC2, ABCC3), the human breast cancer resistance protein (BCRP) and the lung-resistance protein (LRP) [36, 38, 39]. The LRP was initially discovered due to its high expression level in a multidrug-resistant but ABCB1-negative lung cancer cell line  and has been identified in 1995 to be the human major vault protein . Thereby, a first link between the MDR and vaults was established.
MVP has been detected in 78% of 61 human cancer cell lines, and its expression levels correlate with resistance against a variety of MDR-related and unrelated drugs [40, 41]. Furthermore, upregulation of the MVP expression level was determined in multiple human cancer cell lines also on treatment with anticancer agents, including anthracyclins, etoposide and cisplatin, further supporting a putative link between treatment sensitivity and cellular stress adaptation to cytotoxic agent [19, 42–47], and overexpression of MVP is also frequently observed at the early steps of resistance selection [45, 46, 48, 49]. Multiple preclinical studies have corroborated this link between MDR and the MVP expression level in cancer cells, despite some contradicting studies and the fact that the MVP knockout mouse does not show enhanced hypersensitivity to cytostatics. However, this might be due to differential cancer to normal cell line treatment sensitivities or the upregulation of other ABC-transporters or unknown mechanisms of MDR on disruption of functional MVP [11, 50].
Vaults may mediate multi-drug resistance by the transport of drugs away from their subcellular targets, by the extrusion from the nucleus and/or the sequestration of drugs into exocytotic vesicles. Either through these exocytotic vesicles or pump molecules like ABCB1, ABCC1, or breast cancer resistance protein at the plasma membrane, the intracellular potency of these cytotoxic agents will be reduced . Alternatively, the two vault-associated VPARP poly(adenosine diphosphate-ribose) polymerase or TEP-1 may fulfil a protective function of the genome and thereby contribute to a drug-resistance profile. Concomitant operation of several drug resistance mechanisms may often be necessary to cause the phenotype of MDR, and co-expression of MVP and ABCC1 frequently occurs and associates with increased drug resistance levels in MDR-selected tumour cells . Interestingly, up-regulation of MVP could be linked in a series of MDR cell lines with a low and intermediate level of drug resistance with subsequent up-regulation of ABCB1 leading to high levels of drug resistance. These findings point the MVP expression level as a marker for low level of drug resistance, which is clinically more relevant [19, 45, 48]. Furthermore, several studies reported that the expression of MVP is a predictive marker in several tumour types for the response to chemotherapy [52, 53].
Interestingly, human vRNAs produce several small RNAs (svRNAs) by mechanisms different from those in the canonical microRNA (miRNA) pathway. svRNAb downregulates CYP3A4, a key enzyme in drug metabolism. This finding contributes to understand the role of vaults particles in drug resistance, proposing a novel function to vRNAs that may help explain this association .
A novel role for MVP: implications in radiotherapy
In recent years, several findings indicate a link between MVP and vaults not only to drug-resistance based on its putative drug-exporter function, but also to DNA-damage and DNA-damage-repair. MVP transcription and protein levels are increased in response to various DNA-damaging agents, including ionizing radiation (IR) . Interestingly, VPARP and, to lesser extent, TEP-1-deficient mice, have an increased incidence of carcinogen-induced colon tumours . These findings indicate that vaults may play a role in facilitating DNA repair processes.
The high cytotoxicity of IR is mainly based on its ability to induce DNA double strand breaks (DSB) via generation of reactive oxygen species. DSB can be repaired by the two major DSB repair machineries: homologous recombination (HR) and non-homologous end joining (NHEJ). The fact that error-free repair by HR depends on the presence of an intact sister chromatid restricts this pathway to occur during S and G2 phases of the cell cycle. This is, in contrast to error-prone repair by NHEJ which can function throughout the cell cycle and is therefore thought to be the predominant DSB repair pathway in mammalian cells. On the other hand, HR represents the more interesting target to sensitize specifically tumor cells to DNA damaging agents leading to an enhanced therapeutic window.
MVP and vaults have recently been linked to both major DSB-machineries. Ku70 and Ku80 are key proteins in NHEJ, and also play a strong regulatory role in apoptosis through Bax/Bcl-2 interactions [57, 58]. The DNA end-joining protein Ku70 inhibits apoptosis by sequestering Bax away from the mitochondria. However, newly synthesized Bax undergoes ubiquitination, which negatively regulates its proapoptotic function by labeling it for proteasomal degradation. Interestingly, ubiquitinated Bax associates with Ku70, which mediates Bax deubiquitination, simultaneously generating the active form of Bax but sequestered away from the mitochondria . Low expression level of Ku70 might therefore also result in low expression levels of Bax. On the level of NHEJ, an inverse correlation between high MVP and low expression levels of Ku70/80 and pro-apoptotic bax was identified in a cohort of 160 patients with localized cervix carcinoma. Interestingly, low Ku70/80 expression was also associated with upregulated Bcl-2, altered p53, and increased proliferation in this set of patients . Despite the lack of mechanistic insights these data suggest that overexpression of MVP and vaults might be involved in a carcinogenic process with resultant genomic instability due to suppressed NHEJ. Though enhanced proliferative activity, subsequent tumour progression and enhanced treatment resistance requires at the same time an upregulated apoptotic threshold, which might be activated through the suppression of Bax, upregulation of Bcl-2, mutated p53-status and upregulation of the IGF-1R and downstream signalling cascades. Eventually, only molecular dissection of the key signal transduction cascades leading to reduced NHEJ-activity in MVP-overexpressing and MVP-depleted cells will elucidate the association between MVP/vaults, NHEJ and a deregulated apoptotic threshold. Moreover, MVP increases with age both in vitro and in vivo, and that age-related upregulation of MVP facilitates apoptosis resistance of senescent human diploid fibroblasts based on the interaction with c-Jun-mediated downregulation of BCL-2 . Thus, MVP is suggested to be playing an important role in the resistance of senescent fibroblasts to apoptosis by modulation of BCL-2 expression by JNK pathway, regulating cellular signaling and survival and being a potential therapeutic target for modulation of resistance to apoptosis implicated in aging modulation and cancer treatment .
A novel link between the intracellular level of MVP and homologous recombination, the second DNA-DSB-repair machinery, has recently been identified as part of the cellular stress response to ionizing radiation (Presented in ESTRO 29; S 51, 129 oral). MVP-depleted tumour cells are more radiosensitive than their wildtype cognate cells, probably due to direct interference with homologous recombination. In comparison to control cells, Rad51-foci are strongly reduced in irradiated but MVP-depleted cells and thus vaults may coordinate correct HR-complex formation at the site of the DSB. Vaults might also guarantee sufficient protein level of Rad51 required for homologous recombination via coordinated Rad51 expression. It has been reported that MVP mediates nuclear import of PTEN and regulates its nuclear function [24, 62]. Nuclear PTEN expression is high in untransformed cells but decreases with tumour progression. While cytoplasmic PTEN inhibits the phosphoinositol-3-kinase (PI3K)/AKT pathway, nuclear PTEN inhibits phosphorylation of MAPK and consequently reduces the expression of cyclin D leading to a G0/G1 cell cycle arrest. Though, PTEN also acts as cofactor for the transcription factor E2F1, driving expression of RAD51. Cells deficient in PTEN have reduced capacity of homologous recombination-dependent DNA repair, most probably due to reduced levels of RAD51. Thereby, PTEN is also important for the maintenance of chromosomal stability reducing the incidence of spontaneous double strand breaks. Interestingly, loss of PTEN in tumour cells and concomitantly reduced homologous recombination can thereby be exploited by the inhibiton of PARP, creating a situation of synthetic lethality [63, 64]. Since MVP and vaults are involved in nuclear import of PTEN and homologous recombination, it will be of interest to determine whether the MVP expression level also affects genomic instability and sensitivity to PARP-inhibition.
Despite the fact that we have so far only limited insights into the mechanisms how MVP and vault interact with the two double strand-break repair machineries, these results indicate that MVP and vaults may co-regulate correct DNA repair of spontaneous and treatment-induced double strand breaks. Due to the barrel-like structure vaults may act as carriers not only for toxic agents but also for the coordinated intracellular transport of DNA-repair related proteins and thereby act as scaffold protein or shuttling vector. Otherwise, the vaults associated proteins VPARP or TEP1 might play a so far not-identified role for correct DNA repair, which could only be achieved as part of an intact vaults. As indicated above, in vitro studies revealed that gene expression levels of MDR-related proteins increased after fractionated irradiation. It is well known that radiation treatment can induce resistance to various cytotoxic drugs , although the molecular basis of this interaction are complex. Irradiated cell lines showed a significant resistance to cisplatin, doxorubicin and bendamustine, suggesting a novel mechanism in the appearance of MDR, which might involve a radiation-induced increment of LRP/MVP .
MVP and tumour malignancies: vaults as a predictive/prognostic marker
Clinical studies investigating the association between MVP and therapy response and survival
On the other hand, relatively few studies have addressed the role of MVP in solid tumours, with contradictory findings. Thus, the quality of MVP as prognostic marker in ovarian carcinoma is unclear so far, with positive [79, 80] and negative [81, 82] associations reported. Result regarding to breast cancer [83, 84], non-small cell lung cancer [85, 86], or different types of sarcomas are inconclusive [87, 88]. On the other hand, MVP has been established as a reliable factor for response to chemotherapy in bladder cancer patients , melanoma , and determining the aggressive phenotype of testicular germ-cell tumours  and glioblastoma .
With regard to a putative role of MVP in radiation resistance, it has been reported that MVP expression is strongly associated with local-disease free survival and cancer-specific survival in a series of patients suffering from squamous cell carcinoma of the oropharynx who received primary radiotherapy with curative intent (in univariate and multivariate analyses) . Elevated MVP expression seems to be associated with a RT-resistant subset of patients, proposing MVP as a novel useful prognostic marker associated with RT . The underlying mechanisms behind this association are not well defined today but might be linked to the above cited role of MVP in DNA repair and apoptotic threshold.
The complexity but also interest in MVP as a predictive and/or prognostic marker is also illustrated in cervical cancer patients receiving combined radiochemotherapy . Increased MVP and IGF-1R expression levels were related to a small cohort of cervical cancer patients with reduced long-term local control, in patients who otherwise achieved clinical complete response to radiochemotherapy. High MVP expression was strongly related to high IGF1-R expression, which is associated with chemo- and radioresistance in localized cervical carcinoma patients . This association suggests that both proteins need to be expressed to confer chemoradioresistance in cervical cancer. This was further supported by a subgroup of patients who showed low levels of MVP and IGF1R and who presented excellent survival rates once . All of the responding patients with negative or fairly positive (low MVP) tumours were free of local, distant or death related disease. These results were similar to the ones previously obtained for IGF-1R as a prognostic marker . Combination analysis was performed grouping MVP and IGF-1R expression in tumours, showing that all responding patients with low MVP/IGF-1R tumours were free of local disease, relapse or death related disease.
As illustrated by these studies, we are still far away to have identified a mechanistic link between MVP expression level and treatment response, and it is still a matter of debate in how far vaults are involved in chemo- and radioresistance and thus might serve as predictive marker of therapy response. MVP is associated with MDR, and several studies have recognized MVP as a negative prognostic factor for response to chemo- and radiotherapy and/or disease-free survival or overall survival. Molecular, genetic, and clinical data obtained so far warrant further studies into the role of MVP, vaults-related resistance mechanisms.
Vaults are ubiquitous ribonucleoprotein complexes involved in a diversity of cellular processes, including multidrug resistance, transport mechanisms, signal transmission and immune response. Nonetheless, new roles have been assigned to MVP in the field of radiotherapy, where vaults have been proposed as a useful prognostic marker associated with radiotherapy resistance. The molecular mechanisms behind this relevant role are unknown, although a possible association with relevant key players in non-homologous endjoining repair and homologous recombination could exist. Additional translational and clinical studies are required to test this hypothesis.
List of abbreviations
multidrug resistance protein
major vaults protein
non-homologous end joining
vault poly(adenosine diphosphate-ribose) polymerase.
We thank Professor Adrian Begg for helpful comments during the preparation of this manuscript.
- Kedersha NL, Heuser JE, Chugani DC, Rome LH: Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry. J Cell Biol 1991, 112: 225-235. 10.1083/jcb.112.2.225PubMed
- Scheffer GL, Wijngaard PL, Flens MJ, Izquierdo MA, Slovak ML, Pinedo HM, Meijer CJ, Clevers HC, Scheper RJ: The drug resistance-related protein LRP is the human major vault protein. Nat Med 1995, 1: 578-582. 10.1038/nm0695-578PubMed
- Kickhoefer VA, Siva AC, Kedersha NL, Inman EM, Ruland C, Streuli M, Rome LH: The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase. J Cell Biol 1999, 146: 917-928. 10.1083/jcb.146.5.917PubMed CentralPubMed
- Kickhoefer VA, Stephen AG, Harrington L, Robinson MO, Rome LH: Vaults and telomerase share a common subunit, TEP1. J Biol Chem 1999, 274: 32712-32717. 10.1074/jbc.274.46.32712PubMed
- Kedersha NL, Rome LH: Isolation and characterization of a novel ribonucleoprotein particle: large structures contain a single species of small RNA. J Cell Biol 1986, 103: 699-709. 10.1083/jcb.103.3.699PubMed
- Tanaka H, Kato K, Yamashita E, Sumizawa T, Zhou Y, Yao M, Iwasaki K, Yoshimura M, Tsukihara T: The structure of rat liver vault at 3.5 angstrom resolution. Science 2009, 323: 384-388. 10.1126/science.1164975PubMed
- Xia Y, Ramgopal Y, Li H, Shang L, Srinivas P, Kickhoefer VA, Rome LH, Preiser PR, Boey F, Zhang H, Venkatraman SS: Immobilization of recombinant vault nanoparticles on solid substrates. ACS Nano 2010, 4: 1417-1424. 10.1021/nn901167sPubMed
- Yang J, Kickhoefer VA, Ng BC, Gopal A, Bentolila LA, John S, Tolbert SH, Rome LH: Vaults Are Dynamically Unconstrained Cytoplasmic Nanoparticles Capable of Half Vault Exchange. ACS Nano 2010, 4: 7229-7240. 10.1021/nn102051rPubMed CentralPubMed
- Kong LB, Siva AC, Kickhoefer VA, Rome LH, Stewart PL: RNA location and modeling of a WD40 repeat domain within the vault. RNA 2000, 6: 890-900. 10.1017/S1355838200000157PubMed CentralPubMed
- Kedersha NL, Miquel MC, Bittner D, Rome LH: Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes. J Cell Biol 1990, 110: 895-901. 10.1083/jcb.110.4.895PubMed
- Mossink MH, van Zon A, Franzel-Luiten E, Schoester M, Kickhoefer VA, Scheffer GL, Scheper RJ, Sonneveld P, Wiemer EA: Disruption of the murine major vault protein (MVP/LRP) gene does not induce hypersensitivity to cytostatics. Cancer Res 2002, 62: 7298-7304.PubMed
- Slovak ML, Ho JP, Cole SP, Deeley RG, Greenberger L, de Vries EG, Broxterman HJ, Scheffer GL, Scheper RJ: The LRP gene encoding a major vault protein associated with drug resistance maps proximal to MRP on chromosome 16: evidence that chromosome breakage plays a key role in MRP or LRP gene amplification. Cancer Res 1995, 55: 4214-4219.PubMed
- Izquierdo MA, van der Zee AG, Vermorken JB, van der Valk P, Belien JA, Giaccone G, Scheffer GL, Flens MJ, Pinedo HM, Kenemans P, et al.: Drug resistance-associated marker Lrp for prediction of response to chemotherapy and prognoses in advanced ovarian carcinoma. J Natl Cancer Inst 1995, 87: 1230-1237. 10.1093/jnci/87.16.1230PubMed
- Lange C, Walther W, Schwabe H, Stein U: Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter. Biochem Biophys Res Commun 2000, 278: 125-133. 10.1006/bbrc.2000.3782PubMed
- Schroder K, Hertzog PJ, Ravasi T, Hume DA: Interferon-gamma: an overview of signals, mechanisms and functions. J Leukoc Biol 2004, 75: 163-189.PubMed
- Steiner E, Holzmann K, Pirker C, Elbling L, Micksche M, Sutterluty H, Berger W: The major vault protein is responsive to and interferes with interferon-gamma-mediated STAT1 signals. J Cell Sci 2006, 119: 459-469. 10.1242/jcs.02773PubMed
- Izquierdo MA, Scheffer GL, Flens MJ, Giaccone G, Broxterman HJ, Meijer CJ, van der Valk P, Scheper RJ: Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors. Am J Pathol 1996, 148: 877-887.PubMed CentralPubMed
- Kedersha NL, Rome LH: Vaults: large cytoplasmic RNP's that associate with cytoskeletal elements. Mol Biol Rep 1990, 14: 121-122. 10.1007/BF00360441PubMed
- Scheper RJ, Broxterman HJ, Scheffer GL, Kaaijk P, Dalton WS, van Heijningen TH, van Kalken CK, Slovak ML, de Vries EG, van der Valk P, et al.: Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance. Cancer Res 1993, 53: 1475-1479.PubMed
- Chugani DC, Rome LH, Kedersha NL: Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex. J Cell Sci 1993,106(Pt 1):23-29.PubMed
- Hamill DR, Suprenant KA: Characterization of the sea urchin major vault protein: a possible role for vault ribonucleoprotein particles in nucleocytoplasmic transport. Dev Biol 1997, 190: 117-128. 10.1006/dbio.1997.8676PubMed
- Kolli S, Zito CI, Mossink MH, Wiemer EA, Bennett AM: The major vault protein is a novel substrate for the tyrosine phosphatase SHP-2 and scaffold protein in epidermal growth factor signaling. J Biol Chem 2004, 279: 29374-29385. 10.1074/jbc.M313955200PubMed
- Kim E, Lee S, Mian MF, Yun SU, Song M, Yi KS, Ryu SH, Suh PG: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling. FEBS J 2006, 273: 793-804. 10.1111/j.1742-4658.2006.05112.xPubMed
- Yu Z, Fotouhi-Ardakani N, Wu L, Maoui M, Wang S, Banville D, Shen SH: PTEN associates with the vault particles in HeLa cells. J Biol Chem 2002, 277: 40247-40252. 10.1074/jbc.M207608200PubMed
- Chung JH, Ginn-Pease ME, Eng C: Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) has nuclear localization signal-like sequences for nuclear import mediated by major vault protein. Cancer Res 2005, 65: 4108-4116. 10.1158/0008-5472.CAN-05-0124PubMed
- Yi C, Li S, Chen X, Wiemer EA, Wang J, Wei N, Deng XW: Major vault protein, in concert with constitutively photomorphogenic 1, negatively regulates c-Jun-mediated activator protein 1 transcription in mammalian cells. Cancer Res 2005, 65: 5835-5840. 10.1158/0008-5472.CAN-05-0423PubMed
- Iwashita K, Ikeda R, Takeda Y, Sumizawa T, Furukawa T, Yamaguchi T, Akiyama S, Yamada K: Major vault protein forms complexes with hypoxia-inducible factor (HIF)-1alpha and reduces HIF-1alpha level in ACHN human renal adenocarcinoma cells. Cancer Sci 2010, 101: 920-926. 10.1111/j.1349-7006.2009.01481.xPubMed
- Kowalski MP, Dubouix-Bourandy A, Bajmoczi M, Golan DE, Zaidi T, Coutinho-Sledge YS, Gygi MP, Gygi SP, Wiemer EA, Pier GB: Host resistance to lung infection mediated by major vault protein in epithelial cells. Science 2007, 317: 130-132. 10.1126/science.1142311PubMed CentralPubMed
- Mossink MH, de Groot J, van Zon A, Franzel-Luiten E, Schoester M, Scheffer GL, Sonneveld P, Scheper RJ, Wiemer EA: Unimpaired dendritic cell functions in MVP/LRP knockout mice. Immunology 2003, 110: 58-65. 10.1046/j.1365-2567.2003.01708.xPubMed CentralPubMed
- Schroeijers AB, Reurs AW, Scheffer GL, Stam AG, de Jong MC, Rustemeyer T, Wiemer EA, de Gruijl TD, Scheper RJ: Up-regulation of drug resistance-related vaults during dendritic cell development. J Immunol 2002, 168: 1572-1578.PubMed
- Berger W, Steiner E, Grusch M, Elbling L, Micksche M: Vaults and the major vault protein: novel roles in signal pathway regulation and immunity. Cell Mol Life Sci 2009, 66: 43-61. 10.1007/s00018-008-8364-zPubMed
- Motsch N, Pfuhl T, Mrazek J, Barth S, Grasser FA: Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induces the expression of the cellular microRNA miR-146a. RNA Biol 2007, 4: 131-137. 10.4161/rna.4.3.5206PubMed
- Mrazek J, Kreutmayer SB, Grasser FA, Polacek N, Huttenhofer A: Subtractive hybridization identifies novel differentially expressed ncRNA species in EBV-infected human B cells. Nucleic Acids Res 2007, 35: e73. 10.1093/nar/gkm244PubMed CentralPubMed
- Taganov KD, Boldin MP, Chang KJ, Baltimore D: NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA 2006, 103: 12481-12486. 10.1073/pnas.0605298103PubMed CentralPubMed
- Kickhoefer VA, Vasu SK, Rome LH: Vaults are the answer, what is the question? Trends Cell Biol 1996, 6: 174-178. 10.1016/0962-8924(96)10014-3PubMed
- Steiner E, Holzmann K, Elbling L, Micksche M, Berger W: Cellular functions of vaults and their involvement in multidrug resistance. Curr Drug Targets 2006, 7: 923-934. 10.2174/138945006778019345PubMed
- Suprenant KA: Vault ribonucleoprotein particles: sarcophagi, gondolas, or safety deposit boxes? Biochemistry 2002, 41: 14447-14454. 10.1021/bi026747ePubMed
- Hall MD, Handley MD, Gottesman MM: Is resistance useless? Multidrug resistance and collateral sensitivity. Trends Pharmacol Sci 2009, 30: 546-556. 10.1016/j.tips.2009.07.003PubMed CentralPubMed
- Scheffer GL, Schroeijers AB, Izquierdo MA, Wiemer EA, Scheper RJ: Lung resistance-related protein/major vault protein and vaults in multidrug-resistant cancer. Curr Opin Oncol 2000, 12: 550-556. 10.1097/00001622-200011000-00007PubMed
- Izquierdo MA, Shoemaker RH, Flens MJ, Scheffer GL, Wu L, Prather TR, Scheper RJ: Overlapping phenotypes of multidrug resistance among panels of human cancer-cell lines. Int J Cancer 1996, 65: 230-237. 10.1002/(SICI)1097-0215(19960117)65:2<230::AID-IJC17>3.0.CO;2-HPubMed
- Laurencot CM, Scheffer GL, Scheper RJ, Shoemaker RH: Increased LRP mRNA expression is associated with the MDR phenotype in intrinsically resistant human cancer cell lines. Int J Cancer 1997, 72: 1021-1026. 10.1002/(SICI)1097-0215(19970917)72:6<1021::AID-IJC17>3.0.CO;2-7PubMed
- Berger W, Elbling L, Micksche M: Expression of the major vault protein LRP in human non-small-cell lung cancer cells: activation by short-term exposure to antineoplastic drugs. Int J Cancer 2000, 88: 293-300. 10.1002/1097-0215(20001015)88:2<293::AID-IJC23>3.0.CO;2-SPubMed
- Berger W, Spiegl-Kreinecker S, Buchroithner J, Elbling L, Pirker C, Fischer J, Micksche M: Overexpression of the human major vault protein in astrocytic brain tumor cells. Int J Cancer 2001, 94: 377-382. 10.1002/ijc.1486PubMed
- Kickhoefer VA, Rajavel KS, Scheffer GL, Dalton WS, Scheper RJ, Rome LH: Vaults are up-regulated in multidrug-resistant cancer cell lines. J Biol Chem 1998, 273: 8971-8974. 10.1074/jbc.273.15.8971PubMed
- Moran E, Cleary I, Larkin AM, Amhlaoibh RN, Masterson A, Scheper RJ, Izquierdo MA, Center M, O'Sullivan F, Clynes M: Co-expression of MDR-associated markers, including P-170, MRP and LRP and cytoskeletal proteins, in three resistant variants of the human ovarian carcinoma cell line, OAW42. Eur J Cancer 1997, 33: 652-660. 10.1016/S0959-8049(96)00501-1PubMed
- Verovski VN, Van den Berge DL, Delvaeye MM, Scheper RJ, De Neve WJ, Storme GA: Low-level doxorubicin resistance in P-glycoprotein-negative human pancreatic tumour PSN1/ADR cells implicates a brefeldin A-sensitive mechanism of drug extrusion. Br J Cancer 1996, 73: 596-602. 10.1038/bjc.1996.103PubMed CentralPubMed
- Kitazono M, Sumizawa T, Takebayashi Y, Chen ZS, Furukawa T, Nagayama S, Tani A, Takao S, Aikou T, Akiyama S: Multidrug resistance and the lung resistance-related protein in human colon carcinoma SW-620 cells. J Natl Cancer Inst 1999, 91: 1647-1653. 10.1093/jnci/91.19.1647PubMed
- Versantvoort CH, Withoff S, Broxterman HJ, Kuiper CM, Scheper RJ, Mulder NH, de Vries EG: Resistance-associated factors in human small-cell lung-carcinoma GLC4 sub-lines with increasing adriamycin resistance. Int J Cancer 1995, 61: 375-380. 10.1002/ijc.2910610317PubMed
- Wyler B, Shao Y, Schneider E, Cianfriglia M, Scheper RJ, Frey BM, Gieseler F, Schmid L, Twentyman PR, Lehnert M: Intermittent exposure to doxorubicin in vitro selects for multifactorial non-P-glycoprotein-associated multidrug resistance in RPMI 8226 human myeloma cells. Br J Haematol 1997, 97: 65-75. 10.1046/j.1365-2141.1997.52649.xPubMed
- Ikuta K, Takemura K, Sasaki K, Kihara M, Nishimura M, Ueda N, Naito S, Lee E, Shimizu E, Yamauchi A: Expression of multidrug resistance proteins and accumulation of cisplatin in human non-small cell lung cancer cells. Biol Pharm Bull 2005, 28: 707-712. 10.1248/bpb.28.707PubMed
- Mossink MH, van Zon A, Scheper RJ, Sonneveld P, Wiemer EA: Vaults: a ribonucleoprotein particle involved in drug resistance? Oncogene 2003, 22: 7458-7467. 10.1038/sj.onc.1206947PubMed
- Lloret M, Lara PC, Bordon E, Rey A, Falcon O, Apolinario RM, Clavo B, Ruiz A: MVP expression is related to IGF1-R in cervical carcinoma patients treated by radiochemotherapy. Gynecol Oncol 2008, 110: 304-307. 10.1016/j.ygyno.2008.04.034PubMed
- van den Heuvel-Eibrink MM, Sonneveld P, Pieters R: The prognostic significance of membrane transport-associated multidrug resistance (MDR) proteins in leukemia. Int J Clin Pharmacol Ther 2000, 38: 94-110.PubMed
- Persson H, Kvist A, Vallon-Christersson J, Medstrand P, Borg A, Rovira C: The non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs. Nat Cell Biol 2009, 11: 1268-1271. 10.1038/ncb1972PubMed
- Shimamoto Y, Sumizawa T, Haraguchi M, Gotanda T, Jueng HC, Furukawa T, Sakata R, Akiyama S: Direct activation of the human major vault protein gene by DNA-damaging agents. Oncol Rep 2006, 15: 645-652.PubMed
- Raval-Fernandes S, Kickhoefer VA, Kitchen C, Rome LH: Increased susceptibility of vault poly(ADP-ribose) polymerase-deficient mice to carcinogen-induced tumorigenesis. Cancer Res 2005, 65: 8846-8852. 10.1158/0008-5472.CAN-05-0770PubMed
- Amsel AD, Rathaus M, Kronman N, Cohen HY: Regulation of the proapoptotic factor Bax by Ku70-dependent deubiquitylation. Proc Natl Acad Sci USA 2008, 105: 5117-5122. 10.1073/pnas.0706700105PubMed CentralPubMed
- Wang Q, Gao F, May WS, Zhang Y, Flagg T, Deng X: Bcl2 negatively regulates DNA double-strand-break repair through a nonhomologous end-joining pathway. Mol Cell 2008, 29: 488-498. 10.1016/j.molcel.2007.12.029PubMed CentralPubMed
- Lloret M, Lara PC, Bordon E, Fontes F, Rey A, Pinar B, Falcon O: Major vault protein may affect nonhomologous end-joining repair and apoptosis through Ku70/80 and bax downregulation in cervical carcinoma tumors. Int J Radiat Oncol Biol Phys 2009, 73: 976-979. 10.1016/j.ijrobp.2008.11.013PubMed
- Ryu SJ, An HJ, Oh YS, Choi HR, Ha MK, Park SC: On the role of major vault protein in the resistance of senescent human diploid fibroblasts to apoptosis. Cell Death Differ 2008, 15: 1673-1680. 10.1038/cdd.2008.96PubMed
- Ryu SJ, Park SC: Targeting major vault protein in senescence-associated apoptosis resistance. Expert Opin Ther Targets 2009, 13: 479-484. 10.1517/14728220902832705PubMed
- Minaguchi T, Waite KA, Eng C: Nuclear localization of PTEN is regulated by Ca(2+) through a tyrosil phosphorylation-independent conformational modification in major vault protein. Cancer Res 2006, 66: 11677-11682. 10.1158/0008-5472.CAN-06-2240PubMed
- Dedes KJ, Wetterskog D, Mendes-Pereira AM, Natrajan R, Lambros MB, Geyer FC, Vatcheva R, Savage K, Mackay A, Lord CJ, et al.: PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors. Sci Transl Med 2010, 2: 53ra75. 10.1126/scitranslmed.3001538PubMed
- Mendes-Pereira AM, Martin SA, Brough R, McCarthy A, Taylor JR, Kim JS, Waldman T, Lord CJ, Ashworth A: Synthetic lethal targeting of PTEN mutant cells with PARP inhibitors. EMBO Mol Med 2009, 1: 315-322. 10.1002/emmm.200900041PubMed CentralPubMed
- Mitchell JB, Russo A, Cook JA, Glatstein E: Tumor cell drug and radiation resistance: does an interrelationship exist? Cancer Treat Res 1989, 48: 189-203. 10.1007/978-1-4613-1601-5_12PubMed
- Bottke D, Koychev D, Busse A, Heufelder K, Wiegel T, Thiel E, Hinkelbein W, Keilholz U: Fractionated irradiation can induce functionally relevant multidrug resistance gene and protein expression in human tumor cell lines. Radiat Res 2008, 170: 41-48. 10.1667/RR0986.1PubMed
- Filipits M, Pohl G, Stranzl T, Suchomel RW, Scheper RJ, Jager U, Geissler K, Lechner K, Pirker R: Expression of the lung resistance protein predicts poor outcome in de novo acute myeloid leukemia. Blood 1998, 91: 1508-1513.PubMed
- Filipits M, Stranzl T, Pohl G, Heinzl H, Jager U, Geissler K, Fonatsch C, Haas OA, Lechner K, Pirker R: Drug resistance factors in acute myeloid leukemia: a comparative analysis. Leukemia 2000, 14: 68-76. 10.1038/sj.leu.2401634PubMed
- Pirker R, Pohl G, Stranzl T, Suchomel RW, Scheper RJ, Jager U, Geissler K, Lechner K, Filipits M: The lung resistance protein (LRP) predicts poor outcome in acute myeloid leukemia. Adv Exp Med Biol 1999, 457: 133-139. 10.1007/978-1-4615-4811-9_15PubMed
- Xu D, Arestrom I, Virtala R, Pisa P, Peterson C, Gruber A: High levels of lung resistance related protein mRNA in leukaemic cells from patients with acute myelogenous leukaemia are associated with inferior response to chemotherapy and prior treatment with mitoxantrone. Br J Haematol 1999, 106: 627-633. 10.1046/j.1365-2141.1999.01611.xPubMed
- Oh EJ, Kahng J, Kim Y, Kim M, Lim J, Kang CS, Min WS, Cho B, Lee A, Lee KY, et al.: Expression of functional markers in acute lymphoblastic leukemia. Leuk Res 2003, 27: 903-908. 10.1016/S0145-2126(03)00026-2PubMed
- Valera ET, Scrideli CA, Queiroz RG, Mori BM, Tone LG: Multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) gene expression in childhood acute lymphoblastic leukemia. Sao Paulo Med J 2004, 122: 166-171. 10.1590/S1516-31802004000400007PubMed
- Volm M, Stammler G, Zintl F, Koomagi R, Sauerbrey A: Expression of lung resistance-related protein (LRP) in initial and relapsed childhood acute lymphoblastic leukemia. Anticancer Drugs 1997, 8: 662-665. 10.1097/00001813-199708000-00003PubMed
- Ohno N, Tani A, Uozumi K, Hanada S, Furukawa T, Akiba S, Sumizawa T, Utsunomiya A, Arima T, Akiyama S: Expression of functional lung resistance--related protein predicts poor outcome in adult T-cell leukemia. Blood 2001, 98: 1160-1165. 10.1182/blood.V98.4.1160PubMed
- Sakaki Y, Terashi K, Yamaguchi A, Kawamata N, Tokito Y, Mori H, Umehara M, Yoshiyama T, Ohtsubo H, Arimura K, et al.: Human T-cell lymphotropic virus type I Tax activates lung resistance-related protein expression in leukemic clones established from an adult T-cell leukemia patient. Exp Hematol 2002, 30: 340-345. 10.1016/S0301-472X(02)00775-0PubMed
- Filipits M, Drach J, Pohl G, Schuster J, Stranzl T, Ackermann J, Konigsberg R, Kaufmann H, Gisslinger H, Huber H, et al.: Expression of the lung resistance protein predicts poor outcome in patients with multiple myeloma. Clin Cancer Res 1999, 5: 2426-2430.PubMed
- Rimsza LM, Campbell K, Dalton WS, Salmon S, Willcox G, Grogan TM: The major vault protein (MVP), a new multidrug resistance associated protein, is frequently expressed in multiple myeloma. Leuk Lymphoma 1999, 34: 315-324.PubMed
- Schwarzenbach H: Expression of MDR1/P-glycoprotein, the multidrug resistance protein MRP, and the lung-resistance protein LRP in multiple myeloma. Med Oncol 2002, 19: 87-104. 10.1385/MO:19:2:87PubMed
- Brinkhuis M, Izquierdo MA, Baak JP, van Diest PJ, Kenemans P, Scheffer GL, Scheper RJ: Expression of multidrug resistance-associated markers, their relation to quantitative pathologic tumour characteristics and prognosis in advanced ovarian cancer. Anal Cell Pathol 2002, 24: 17-23.PubMed
- Wang W, Ke S, Chen G, Gao Q, Wu S, Wang S, Zhou J, Yang X, Lu Y, Ma D: Effect of lung resistance-related protein on the resistance to cisplatin in human ovarian cancer cell lines. Oncol Rep 2004, 12: 1365-1370.PubMed
- Arts HJ, Katsaros D, de Vries EG, Massobrio M, Genta F, Danese S, Arisio R, Scheper RJ, Kool M, Scheffer GL, et al.: Drug resistance-associated markers P-glycoprotein, multidrug resistance-associated protein 1, multidrug resistance-associated protein 2, and lung resistance protein as prognostic factors in ovarian carcinoma. Clin Cancer Res 1999, 5: 2798-2805.PubMed
- Mayr D, Pannekamp U, Baretton GB, Gropp M, Meier W, Flens MJ, Scheper R, Diebold J: Immunohistochemical analysis of drug resistance-associated proteins in ovarian carcinomas. Pathol Res Pract 2000, 196: 469-475. 10.1016/S0344-0338(00)80048-5PubMed
- Burger H, Foekens JA, Look MP, Meijer-van Gelder ME, Klijn JG, Wiemer EA, Stoter G, Nooter K: RNA expression of breast cancer resistance protein, lung resistance-related protein, multidrug resistance-associated proteins 1 and 2, and multidrug resistance gene 1 in breast cancer: correlation with chemotherapeutic response. Clin Cancer Res 2003, 9: 827-836.PubMed
- Schneider J, Lucas R, Sanchez J, Ruibal A, Tejerina A, Martin M: Modulation of molecular marker expression by induction chemotherapy in locally advanced breast cancer: correlation with the response to therapy and the expression of MDR1 and LRP. Anticancer Res 2000, 20: 4373-4377.PubMed
- Berger W, Setinek U, Hollaus P, Zidek T, Steiner E, Elbling L, Cantonati H, Attems J, Gsur A, Micksche M: Multidrug resistance markers P-glycoprotein, multidrug resistance protein 1, and lung resistance protein in non-small cell lung cancer: prognostic implications. J Cancer Res Clin Oncol 2005, 131: 355-363. 10.1007/s00432-004-0653-9PubMed
- Chiou JF, Liang JA, Hsu WH, Wang JJ, Ho ST, Kao A: Comparing the relationship of Taxol-based chemotherapy response with P-glycoprotein and lung resistance-related protein expression in non-small cell lung cancer. Lung 2003, 181: 267-273. 10.1007/s00408-003-1029-7PubMed
- Komdeur R, Klunder J, van der Graaf WT, van den Berg E, de Bont ES, Hoekstra HJ, Molenaar WM: Multidrug resistance proteins in rhabdomyosarcomas: comparison between children and adults. Cancer 2003, 97: 1999-2005. 10.1002/cncr.11259PubMed
- Komdeur R, Plaat BE, van der Graaf WT, Hoekstra HJ, Hollema H, van den Berg E, Zwart N, Scheper RJ, Molenaar WM: Expression of multidrug resistance proteins, P-gp, MRP1 and LRP, in soft tissue sarcomas analysed according to their histological type and grade. Eur J Cancer 2003, 39: 909-916. 10.1016/S0959-8049(03)00029-7PubMed
- Diestra JE, Condom E, Del Muro XG, Scheffer GL, Perez J, Zurita AJ, Munoz-Segui J, Vigues F, Scheper RJ, Capella G, et al.: Expression of multidrug resistance proteins P-glycoprotein, multidrug resistance protein 1, breast cancer resistance protein and lung resistance related protein in locally advanced bladder cancer treated with neoadjuvant chemotherapy: biological and clinical implications. J Urol 2003, 170: 1383-1387. 10.1097/01.ju.0000074710.96154.c9PubMed
- Schadendorf D, Makki A, Stahr C, van Dyck A, Wanner R, Scheffer GL, Flens MJ, Scheper R, Henz BM: Membrane transport proteins associated with drug resistance expressed in human melanoma. Am J Pathol 1995, 147: 1545-1552.PubMed CentralPubMed
- Mandoky L, Geczi L, Doleschall Z, Bodrogi I, Csuka O, Kasler M, Bak M: Expression and prognostic value of the lung resistance-related protein (LRP) in germ cell testicular tumors. Anticancer Res 2004, 24: 1097-1104.PubMed
- Tews DS, Nissen A, Kulgen C, Gaumann AK: Drug resistance-associated factors in primary and secondary glioblastomas and their precursor tumors. J Neurooncol 2000, 50: 227-237. 10.1023/A:1006491405010PubMed
- Silva P, West CM, Slevin N, Valentine H, Ryder WD, Hampson L, Bibi R, Sloan P, Thakker N, Homer J, Hampson I: Tumor expression of major vault protein is an adverse prognostic factor for radiotherapy outcome in oropharyngeal carcinoma. Int J Radiat Oncol Biol Phys 2007, 69: 133-140. 10.1016/j.ijrobp.2007.02.025PubMed
- Lloret M, Lara PC, Bordon E, Pinar B, Rey A, Falcon O, Molano F, Hernandez MA: IGF-1R expression in localized cervical carcinoma patients treated by radiochemotherapy. Gynecol Oncol 2007, 106: 8-11. 10.1016/j.ygyno.2007.04.004PubMed
- Ramani P, Dewchand H: Expression of mdr1/P-glycoprotein and p110 in neuroblastoma. J Pathol 1995, 175: 13-22. 10.1002/path.1711750104PubMed
- Dingemans AM, van Ark-Otte J, van der Valk P, Apolinario RM, Scheper RJ, Postmus PE, Giaccone G: Expression of the human major vault protein LRP in human lung cancer samples and normal lung tissues. Ann Oncol 1996, 7: 625-630.PubMed
- Linn SC, Pinedo HM, van Ark-Otte J, van der Valk P, Hoekman K, Honkoop AH, Vermorken JB, Giaccone G: Expression of drug resistance proteins in breast cancer, in relation to chemotherapy. Int J Cancer 1997, 71: 787-795. 10.1002/(SICI)1097-0215(19970529)71:5<787::AID-IJC16>3.0.CO;2-5PubMed
- Uozaki H, Horiuchi H, Ishida T, Iijima T, Imamura T, Machinami R: Overexpression of resistance-related proteins (metallothioneins, glutathione-S-transferase pi, heat shock protein 27, and lung resistance-related protein) in osteosarcoma. Relationship with poor prognosis. Cancer 1997, 79: 2336-2344. 10.1002/(SICI)1097-0142(19970615)79:12<2336::AID-CNCR7>3.0.CO;2-JPubMed
- Pohl G, Filipits M, Suchomel RW, Stranzl T, Depisch D, Pirker R: Expression of the lung resistance protein (LRP) in primary breast cancer. Anticancer Res 1999, 19: 5051-5055.PubMed
- Volm M, Rittgen W: Cellular predictive factors for the drug response of lung cancer. Anticancer Res 2000, 20: 3449-3458.PubMed
- Goff BA, Paley PJ, Greer BE, Gown AM: Evaluation of chemoresistance markers in women with epithelial ovarian carcinoma. Gynecol Oncol 2001, 81: 18-24. 10.1006/gyno.2000.6105PubMed
- Pohl G, Suchomel RW, Stranzl T, Depisch D, Stiglbauer W, Filipits M, Pirker R: Expression of the lung resistance protein in primary colorectal carcinomas. Anticancer Res 2001, 21: 201-204.PubMed
- Harada T, Ogura S, Yamazaki K, Kinoshita I, Itoh T, Isobe H, Yamashiro K, Dosaka-Akita H, Nishimura M: Predictive value of expression of P53, Bcl-2 and lung resistance-related protein for response to chemotherapy in non-small cell lung cancers. Cancer Sci 2003, 94: 394-399. 10.1111/j.1349-7006.2003.tb01453.xPubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.